Abeomics/CpG ODN（1668），TLR9配体（B类）/1 mg/15-1019-免疫在线蚂蚁淘旗下平台

商品详细Abeomics/CpG ODN（1668），TLR9配体（B类）/1 mg/15-1019

Abeomics/CpG ODN（1668），TLR9配体（B类）/1 mg/15-1019

商品编号： 15-1019

品牌： Abeomics

市场价： ¥7980.00

美元价： 3990.00

产地：美国(厂家直采)

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产品分类：酶抑制剂

公司分类： Enzyme_Inhibitors

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Amount :	0.1 mg
Content :	1 mg/ml in endotoxin-free water
Storage condition :	Upon receipt, store at -20°C (Stable for at least 6 months). Avoid frequent freeze/thaw cycles.

Sequence:5'-tccatgaCGttcctgatgct-3' (Class B ODN 1668: Bases are phosphorothioate.)

Synthetic oligodeoxynucleotides (ODN) containing unmethylated deoxycytosine-deoxyguanosine (CpG) motif are equivalent to bacterial DNA in the immunostimulatory activity, which can induce innate immunity via Toll-like receptor 9 (TLR9) in mammals. There are three major classes of CpG ODNs, and each ODN class exhibits different stimulatory effects on immune cell activation. Class A ODNs are potent inducers of IFN-alpha that leads to the plasmacytoid dendritc cell (pDC) maturation. Class B ODNs are relatively weak inducers of type I IFNs but strong stimulators of human B cells and monocyte maturation. Class C ODNs combine elements of both Classes A and B ODNs, which can induce IFN-alpha in pDC and activation of B cells.

A. CpG ODN 1668-mediated mouse TLR9 activation in NF-kBLeeporter™– RAW 264.7 cells (Figure 2).

1. Harvest NF-kB Leeporter™ – RAW 264.7 cells and seed cells into a white solid-bottom 96-well microplate in 100 ul of growth medium at 5 x 10⁴cells/well.

2. Incubate cells at 37^oC in a CO₂incubator for overnight.

3. The next day, stimulate cells with various amounts of CpG ODN 1668.

4. Incubate at 37^oC in a CO₂incubator for 6-16 hours.

5. Add 30-50 ul of luciferase assay reagent per well.

6. Incubate at room temperature for 1-5 minutes and measure luminescence using a microplate luminometer.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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