Abeomics/PD-L1稳定细胞系/营利性/14-501ACL-免疫在线蚂蚁淘旗下平台

商品详细Abeomics/PD-L1稳定细胞系/营利性/14-501ACL

Abeomics/PD-L1稳定细胞系/营利性/14-501ACL

商品编号： 14-501ACL

品牌： Abeomics

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：免疫组化试剂盒

公司分类： Immunostain_SP_Kit

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Amount :	Non Profit

PD-L1 Stable Cell Line is a stably transfected CHO-K1 cell line which expresses human Programmed Death- Ligand 1 (PD-L1, also known as CD274 and B7-H1).

Sequence data: hPD-L1 (accession numberNP_054862)

MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEME

DKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGG

ADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTT

TTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTH

LVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET

Content :	Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :	Immediately upon receipt, store in liquid nitrogen.

Application:.

Screen for antibodies of human PD-L1 through Flow Cytometry.

Culture conditions:

Cells should be grown at 37^oC with 5%CO₂using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 10µg/ml of Blasticidin.

It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37^oCwater-bath, transfer to a tube containing 10 ml of growth medium without Blasticidin, spin down cells, resuspend cells in pre-warmed growth medium without Blasticidin, transfer resuspended cells to T25 flask and culture in37^oC-CO₂incubator.

Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation.At first passage, switch to growth medium containing Blasticidin. Cells should be split before they reach complete confluence.

To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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