安倍经济学/关税及贸易总协定™ 荧光素酶报告基因-HEK293细胞系/营利性/14-109ACL-免疫在线蚂蚁淘旗下平台

商品详细安倍经济学/关税及贸易总协定™ 荧光素酶报告基因-HEK293细胞系/营利性/14-109ACL

安倍经济学/关税及贸易总协定™ 荧光素酶报告基因-HEK293细胞系/营利性/14-109ACL

商品编号： 14-109ACL

品牌： Abeomics

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：稳转细胞株

公司分类： Stable_cell_line

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Description
Product Info
Application Note
Review (0)


Amount :	Non Profit

The GATA3 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the GATA3 response element, so that the cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.

Content :	Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :	Immediately upon receipt, store in liquid nitrogen.

Application:

Monitor the GATA3 signaling pathway activity.
Screen for activators or inhibitors of the GATA3 signaling pathway.

Culture conditions:

Cells should be grown at 37^oC with 5%CO₂using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3µg/ml of Puromycin(Note: Puromycin can be omitted during the reporter cell assays).

It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37^oCwater-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in37^oC-CO₂incubator.

Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.

To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly.To achieve satisfactory results, cells should not be passaged over 16 times.

Functional validation:

A. Response of GATA3 Leeporter™ – HEK293 cells to phorbol 12-myristate 13-acetate (PMA).

1. Harvest GATA3 Leeporter™ – HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100µl of growth medium at 5 x 10^4 cells/well.

2. Incubate cells at 37^oC in a CO₂ incubator for overnight.

3. The next day, stimulate cells with different concentrations of PMA.

4. Incubate at 37^oC in a CO₂ incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

There are currently no product reviews

Write a review on this product!