安倍经济学/AP-1™ 荧光素酶报告基因-HEK293细胞系/营利性/14-102ACL-免疫在线蚂蚁淘旗下平台

商品详细安倍经济学/AP-1™ 荧光素酶报告基因-HEK293细胞系/营利性/14-102ACL

安倍经济学/AP-1™ 荧光素酶报告基因-HEK293细胞系/营利性/14-102ACL

商品编号： 14-102ACL

品牌： Abeomics

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：温控控制

公司分类： Temperature_control

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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Amount :	Non Profit

The AP-1 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the activator protein 1 (AP-1). The AP-1 transcription factors are homo- or hetero-dimers that consist of proteins belonging to a group of structurally and functionally related members of the Jun family (c-Jun, JunB and JunD), the Fos (c-Fos, FosB, Fra-1 and Fra-2) and the subfamilies of ATF (ATFa, ATF-2 and ATF-3) and JDP (JDP-1 and JDP-2). The AP-1 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.

Content :	Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
Storage condition :	Immediately upon receipt, store in liquid nitrogen.

Application:

Monitor the AP-1 signaling pathway activity.
Screen for activators or inhibitors of the AP-1 signaling pathway.

Culture conditions:

Cells should be grown at 37^oC with 5%CO₂using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3µg/ml of Puromycin(Note: Puromycin can be omitted during the reporter cell assays).

It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a37^oCwater-bath, transfer to a tube containing 10 ml of growth medium without Puromycin, spin down cells, resuspend cells in pre-warmed growth medium without Puromycin, transfer resuspended cells to T25 flask and culture in37^oC-CO₂incubator.

Leave the T25 flask in the incubator for 1~3 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Puromycin. Cells should be split before they reach complete confluence.

To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.

Functional validation:

A. Response of AP-1 Leeporter™ –HEK293 cells to phorbol 12-myristate 13-acetate (PMA)

1. Harvest AP-1 Leeporter™ –HEK293 cells and seed cells into a white solid-bottom 96-well microplate in 100µlof growth medium at 5 x 10⁴ cells/well.

2. Incubate cells at37^oCin aCO₂incubator for overnight.

3. The next day, stimulate cells with different concentrations of PMA.

4. Incubate at37^oCin aCO₂incubator for 16 hours.

5. Equilibrate the plate to room temperature for 10 minutes.

6. Add 50µl of luciferase assay reagent (Abeomics, Cat #17-1101; Refer to the reagent datasheet for the detailed luciferase assay protocol) per well.

7. Read the plate in 1-5 minutes to measure luminescence using a microplate luminometer.

LIMITED USE RESTRICTIONS:

THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.

By use of this product, user agrees to be bound by the terms of this limited use statement.

This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.

Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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