Format : | Purified |
Amount : | 100 µg |
Isotype : | Mouse IgG1, kappa |
Purification : | Purified Ab with BSA and Azide at 200ug/ml |
Content : | 200ug/ml of recombinant MAb purified Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
Gene : | EPCAM |
Gene ID : | 4072 |
Uniprot ID : | P16422 |
Alternative Name : | Adenocarcinoma-associated Antigen,Cell Surface Glycoprotein Trop-1,EGP2,EGP314,EGP40,Epithelial Cell Adhesion Molecule,Epithelial Glycoprotein 314,ESA,KSA,TACD1,TROP1,Tumor-associated Calcium Signal Transducer 1 (TACSTD1),ECS-1,Epidermal Surface Antigen 1,ESA1,FLOT2,Flotillin-2,Membrane Component,Chromosome 17,Surface Marker-1 (M17S1),REG-1,Reggie-1,Reggie-2 |
Immunogen Information : | Small cell lung carcinoma cells |
This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Does not react with rat and ferret.
MW : 40-43kDa; Positive Control : HT29 cells or breast tumor;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Lateral cell membrane, Cell junction |
Post transnational modification: | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
BioGrid: | 110250.5 interactions. |
There are currently no product reviews Write a review on this product! |